1. What is Relative Centrifugal Force?

Relative Centrifugal Force (RCF) is the measurement of the gravitational force applied in a centrifuge. It provides a standardized way to compare centrifugation conditions across different centrifuges with varying rotor sizes and speeds.

2. How Does the Calculator Work?

The calculator uses the RCF formula:

Where:

• \( RCF \) — Relative centrifugal force (g)
• \( r \) — Radius from center of rotation to sample (cm)
• \( RPM \) — Rotations per minute (rev/min)
• \( 1.118 \times 10^{-5} \) — Conversion constant

Explanation: The formula converts rotational speed (RPM) to gravitational force (g-force) based on the radius of the centrifuge rotor.

3. Importance of RCF Calculation

Details: Accurate RCF calculation is essential for reproducible centrifugation protocols, ensuring consistent separation results across different laboratory equipment and experimental conditions.

4. Using the Calculator

Tips: Enter RPM in revolutions per minute and radius in centimeters. Both values must be positive numbers greater than zero for accurate calculation.

5. Frequently Asked Questions (FAQ)

Q1: Why use RCF instead of RPM?
A: RCF provides a standardized measure of centrifugal force that accounts for rotor radius, making protocols reproducible across different centrifuges.

Q2: What is the typical range for RCF values?
A: RCF values range from 100-20,000 g for most laboratory applications, with ultracentrifuges reaching up to 1,000,000 g.

Q3: How do I measure the radius?
A: Measure from the center of the rotor to the middle of the sample tube when placed in the centrifuge.

Q4: Are there limitations to this calculation?
A: The calculation assumes uniform circular motion and may not account for acceleration/deceleration phases or non-ideal rotor conditions.

Q5: Can I convert RCF back to RPM?
A: Yes, the formula can be rearranged: \( RPM = \sqrt{\frac{RCF}{1.118 \times 10^{-5} \times r}} \)