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Mass Resolution Formula:
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Mass resolution in mass spectrometry is defined as the ability to distinguish between two ions with similar mass-to-charge ratios. It is calculated as the mass divided by the mass difference, providing a dimensionless value that indicates the instrument's separation capability.
The calculator uses the mass resolution formula:
Where:
Explanation: Higher resolution values indicate better separation between peaks, allowing for more precise mass measurements and better identification of compounds.
Details: Mass resolution is crucial in mass spectrometry for accurate compound identification, isotopic pattern recognition, and distinguishing between closely related molecular species in complex mixtures.
Tips: Enter mass and mass difference in Daltons (Da). Both values must be positive numbers greater than zero for accurate calculation.
Q1: What is considered good resolution in mass spectrometry?
A: Good resolution depends on the application. For small molecules, 10,000-30,000 is often sufficient, while proteomics may require 60,000-240,000 or higher.
Q2: How does resolution affect mass accuracy?
A: Higher resolution typically leads to better mass accuracy as it allows for more precise peak definition and centroid determination.
Q3: What factors influence mass resolution?
A: Instrument design, magnetic field strength, analyzer type (Orbitrap, TOF, quadrupole), and operating conditions all affect resolution capabilities.
Q4: Can resolution be too high?
A: Extremely high resolution may reduce sensitivity and increase analysis time, so optimal resolution balances separation needs with practical considerations.
Q5: How is resolution different from resolving power?
A: Resolution is the actual measured value (M/ΔM), while resolving power refers to the instrument's inherent capability to achieve separation.