HPLC Method Transfer Calculator Waters

Waters Column Scaling Equation:

\[ \text{Adjusted Flow} = \text{Original Flow} \times \left( \frac{L_{\text{new}}}{L_{\text{old}}} \right) \times \sqrt{\frac{d_{\text{old}}}{d_{\text{new}}}} \]

Original Flow:

mL/min

L_new:

L_old:

d_new:

µm

d_old:

µm

Adjusted Flow:

Unit Converter ▲

Unit Converter ▼

From:	To:

1. What is HPLC Method Transfer?

HPLC method transfer involves adapting chromatographic methods from one column or instrument to another while maintaining method performance. Waters-specific column scaling ensures consistent separation when changing column dimensions.

2. How Does the Calculator Work?

The calculator uses the Waters column scaling equation:

\[ \text{Adjusted Flow} = \text{Original Flow} \times \left( \frac{L_{\text{new}}}{L_{\text{old}}} \right) \times \sqrt{\frac{d_{\text{old}}}{d_{\text{new}}}} \]

Where:

\( \text{Original Flow} \) — Original flow rate (mL/min)
\( L_{\text{new}} \) — New column length (mm)
\( L_{\text{old}} \) — Original column length (mm)
\( d_{\text{new}} \) — New column particle size (µm)
\( d_{\text{old}} \) — Original column particle size (µm)

Explanation: This equation maintains linear velocity and pressure relationships when scaling between different column geometries, ensuring consistent chromatographic performance.

3. Importance of Method Transfer

Details: Proper method transfer is crucial for maintaining method robustness, ensuring regulatory compliance, and achieving consistent results across different laboratories and equipment.

4. Using the Calculator

Tips: Enter all values in the specified units. Ensure all measurements are accurate and use consistent units throughout. All values must be positive numbers.

5. Frequently Asked Questions (FAQ)

Q1: Why use Waters-specific scaling?
A: Waters columns have specific characteristics that may differ from other manufacturers, making manufacturer-specific scaling more accurate.

Q2: What if I'm changing column diameter?
A: For diameter changes, additional flow rate adjustments are needed based on the square of the diameter ratio.

Q3: How does particle size affect the calculation?
A: Smaller particle sizes require higher pressures but can provide better resolution and efficiency.

Q4: Can this calculator be used for UPLC methods?
A: Yes, the same principles apply, but pressure limitations of your UPLC system should be considered.

Q5: What about gradient time adjustments?
A: Gradient times should be scaled proportionally to the column length ratio to maintain equivalent separation.